Page 28 - Dr Stephanie Seneff - Reviewing Some Possible Unintended Consequences of the mRNA Vaccines Against COVID - 19

2. Permanent DNA integration of Exogenous Retrovirus Genes
Humans are colonized by a large collection of exogenous retroviruses that in many cases cause no
harm to the host, and may even be symbiotic (Luganini and Gribaudo, 2020). Exogenous viruses can
be converted to endogenous viruses (permanently incorporated into host DNA) in the laboratory, as
demonstrated by Rudolf Jaenisch (Jaenisch, 1976), who infected preimplantation mouse embryos
with the Moloney murine leukemia virus (M-MuLV). The mice generated from these infected
embryos developed leukemia, and the viral DNA was integrated into their germ line and transmitted
to their offspring. Besides the incorporation of viral DNA into the host genome, it was also shown
as early as 1980 that DNA plasmids could be microinjected into the nuclei of mouse embryos to
produce transgenic mice that breed true (Gordon et al., 1980). The plasmid DNA was incorporated
into the nuclear genome of the mice through existing natural processes, thus preserving the newly
acquired genetic information in the offspring’s genome. This discovery has been the basis for many
genetic engineering experiments on transgenic mice engineered to express newly acquired human
genes since then (Bouabe and Okkenhaug, 2013).

3. LINE-1 is Widely Expressed
LINEs alone make up over 20% of the human genome. The most common LINE is LINE-1, which
encodes a reverse transcriptase that regulates fundamental biological processes. LINE-1 is expressed
in many cell types, but at especially high levels in sperm. Sperm cells can be used as vectors of both
exogenous DNA and exogenous RNA molecules through sperm-mediated gene transfer assays.
Sperm can reverse transcribe exogenous RNA directly into cDNA and can deliver plasmids
packaging up this cDNA to the fertilized egg. These plasmids are able to propagate themselves
within the developing embryo and to populate many tissues in the fetus. In fact, they survive into
adulthood as extrachromosomal structures and are capable of being passed on to progeny. These
plasmids are transcriptionally competent, meaning that they can be used to synthesize proteins
encoded by the DNA they contain (Pittoggi et al., 2006).
In addition to sperm, embryos also express reverse transcriptase prior to implantation, and its
inhibition causes developmental arrest. LINE-1 is also expressed by cancer cells, and RNA
interference-mediated silencing of human LINE-1 induces differentiation in many cancer cell lines.
Reverse-transcriptase machinery is implicated in the genesis of new genetic information, both in
cancer cells and in germ cells. Many tumor tissues have been found to express high levels of LINE-
1, and to contain many extrachromosomal plasmids in their nucleus. Malignant gliomas are the
primary tumors of the central nervous system. It has been shown experimentally that these tumors
release exosomes containing DNA, RNA and proteins, that end up in the general circulation (Vaidya
and Sugaya, 2020). LINE-1 is also highly expressed in immune cells in several autoimmune diseases
such as systemic lupus erythematosus, Sjögrens and psoriasis (Zhang et al., 2020).

4. Integrating Spike Protein Gene into Human Genome
Remarkably, it has been demonstrated that neurons from the brain of Alzheimer’s patients harbor
multiple variants of the gene for amyloid precursor protein APP, incorporated into the genome,
which are created through a process called somatic gene recombination (SGR) (Kaeser et al., 2020).
SGR requires gene transcription, DNA strand-breaks, and reverse transcriptase activity, all of which

International Journal of Vaccine Theory, Practice, and Research 2(1), May 10, 2021 Page | 416